Sasa borealis의 Diastereomeric 성분들의 역상 고속액체크로마토그래프 분석방법

• 영문명: Reverse-Phase HPLC Method for Identification of Diastereomeric Constituents from Sasa borealis
• 발행기관: 대한약학회
• 저자명: 정연희(Yeon Hee Jeong) 이준(Jun Lee) 권영주(Young Joo Kwon) 서은경(Eun Kyung Seo)
• 간행물 정보: 『약학회지』제50권 제1호 (2006년), 21~25쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.02.28

영문 초록

Reiterated normal-phase column chromatography lead to the isolation and purification of six known compounds but for the first time from the whole plant of Sasa borealis (Hack.) Makino (Gramineae); tricin 4'-O-(erythro-β-guaia-cylglyceryl) ether (1), tricin 4'-O-(threo-β-guaiacylglyceryl) ether (2), tricin 4'-O-[erythro-β-guaiacyl-(9"-O-acetyl)-glyceryl] ether (3), tricin 4'-O-[threo-β-guaiacyl-(9"-O-acetyl)-glyceryl] ether (4), (-)-pinoresinol (5), and vanillin (6). The structures of the compounds (1-6) were established based on interpretation of high resolution NMR (COSY, HSQC, HMBC, and NOESY) spectral data. In particular, compounds 1 and 3 were diastereomers of compounds 2 and 4, respectively. These two sets of diastereomers were able to be simultaneously identified and quantified by a gradient reversed-phase HPLC method with UV photodiode array. This sensitive HPLC method is noteworthy as a simultaneous separetion and identification method to test the extract of the family Gramineae which contains these compounds.

키워드

Sasa borealis Gramineae simultaneous isolation and identification by HPLC