Protoberberine 알칼로이드가 PCl2 세포중의 L-DOPA 유도 세포독성 작용에 미치는 영향

• 영문명: Effects of Protoberberine Alkaloids on L-DOPA-Induced Cytotoxicity in PC12 Cells
• 발행기관: 대한약학회
• 저자명: 이재준(Jae Joon Lee) 김유미(Yu Mi Kim) 김춘매(Chun Mei Jin) 양유정(Yoo Jung Yang) 강민희(Min Hee Kang) 이명구(Myung Koo Lee)
• 간행물 정보: 『약학회지』제47권 제4호 (2003년), 230~233쪽, 전체 4쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2003.08.30

영문 초록

Previously, Protoberberine alkaloids such as berberine and palmatine have been found to lower dopamine content in PCl2 cells (Shin et al., 2000). In this study, the effects of berberine and palmatine on L-DOPA-induced increase in dopamine level and cytotoxicity in PCl2 cells were investigated. Treatment of PCl2 with L-DOPA at concentration ranges of 20~50 μM increased dopamine content and the increase in dopamine levels by L-DOPA was inhibited by 10-40 μM berberine and 10~80 μM palmatine, which the concentration ranges did not show a cytotoxicit7. However, berberine and palmatine at concentrations higher than 50 μM and 100 μM caused a cytotoxicity respectively, In addition, berberine (10~20 μM) and palmatine (10~50 μ M) at non-cytotoxic concentration ranges ageavated L-DOPA-induced cytotoxicity in PCl2 cells (L-DOPA concentration ranges,20~50 μM). The L-DOPA-induced cytotoxicity was also siglificantly potentiated by berberine (50 μM) and palmatine (100 μM) with cytotoxic ranges. These data demonstrate that berberine and palmatine inhibit L-DOPA- induced increase in dopamine content and stimulate L-DOPA-induced neurotoxicity Therefore, the portsibility that the long-term L-DOPA treated patients with berberine and palmatine could be checked the adverse symptoms.

키워드

berberine palmatine L-DOPA-induced cytotoxicity PC12 cells.