Bl6 Melanoma세포에서 Protein Kinase억제제들이 Cyclic AMP 경로를 통한 멜라닌 생성에 미치는 영향

• 영문명: Effects of Protein Kinase Inhibitors on Melanin Production in Bl6 Melanoma Cells Stimulated via Cyclic AMP-dependent Pathway
• 발행기관: 대한약학회
• 저자명: 차상복(Sang Bok Cha) 조남영(Nam Young Cho) 윤미연(Mi Yun Yoon) 임혜원(Hye Won Lim) 김경원(Kyoung Won Kim) 박영미(Young Mi Park) 이지윤(Ji Yun Lee) 이진희(Jin Hee Lee) 김창종(Chang Jong Kim) 심상수(Sang Soo Sim)
• 간행물 정보: 『약학회지』제47권 제1호 (2003년), 31~36쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2003.02.28

영문 초록

To investigate the effect of protein klnase on melanin production via CAMP-dependent pathway, we measured the melanin amount and tyrosinase activity in Bl6 melanoma cells stimulated by alpha-melanocyte stimulating hormone (MSH), forskolin and 8-Br-Camp MSH, forskolin and 8-Br-Camp significantly increased both melanin production and tyrosinase activity in Bl6 cells. Melanin production and tyrosinase activity by MSH are significantly inhibited by cyclic AMP-dependent protein kinase inhibitor (KT5720) and protein kinase C down-regulation treated with PMA. Bisindolmaleimide (1μM), protein kinase C inhibitor, significantly inhibited melanin production and tyrosinase activity stimulated by MSH, for-skolin and 8-Br-Camp with the following order of potency: MSH>forskolin>8-Br-Camp. Tyrosine kinase inhibitor, genistein and DHC, significantly inhibited both, but the inhibitory effect was more potent in 8-Br-Camp-stimulated Bl6 cells than MSH-stimulated cells. NFkB inhibitor (parthenolide) significantly inhibited melanin production and tyrosinase activity. Neither melanin production nor tyrosinase activity induced by MSH, forskolin and 8-Br-Camp were affected by KN-62 (calm-odulin-dependent protein kinase II inhibitor), PD098059 (mitogen-activated protein kinase inhibitor, MAPKK) and worthmannin (phosphatidylinositol 3-kinase inhibitor). These results suggest that both protein klnase C and tyrosine kinase are involved in melanin production by cyclic AMP-dependent pathway and NFrB pathway may play an important role in cyclic AMP-dependent melanin production in Bl6 melanoma cells.

키워드

Melanin tyrosinase protein kinase inhibitors.