마늘의 조직 센서를 이용한 L-asparagine의 정량

• 영문명: Determination of L-asparagine Using A Garlic Tissue Electrode
• 발행기관: 대한약학회
• 저자명: 김성진(Sung Jin Kim) 김기묘(Gi Myou Kim) 배유진(You Jin bae) 이은엽(Eun Yup Lee) 허문회(Moon Hye Hur) 안문규(Moon Kyu Ahn)
• 간행물 정보: 『약학회지』제39권 제2호 (1995년), 113~117쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1995.04.28

영문 초록

Garlic tissue cells are employed for the conversion of L-asparagine into ammonia. An ammonia gas electrode is used as a detector. The effect of pH, buffer solution, temperature and life time of electrode to have used were investigated in order to optimize the electrode response. The combination of L-asparaginase in garlic tissue cells and the gas electrode response linearly to L-asparagine over the concentration range 1.0X10-4 ~ 1.0X10-1M with a slope of 72.0mV/decade and is selective with respect to other L-amino acids.