녹두로부터 렉틴 성분의 분리 정제

• 영문명: Isolation and Purification of Lectin from Phaseolus radiatus
• 발행기관: 대한약학회
• 저자명: 정시련(See Ryun Chung) 홍승수(Seoung Soo Hong) 전경희(Kyung Hee Jeune-Chung)
• 간행물 정보: 『약학회지』제27권 제3호 (1983년), 221~227쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1983.09.30

영문 초록

New lectins, lymphoagglutinating lectins from mung beans (MBLA) have been isolated and purified. Mung beans crude extracts were made with 0.15M NaCl and these were purified through anionic exchange chromatography. Four fractions were obtained from DEAE Sephadex A-50 by salt gradients elution. Lectin activity, enzvme activity, protein assay, identification of purity by Polyacrylamide gel electrphoresis and immunochemical studies were carried out with these four fractions. Through these results, it can be suggested that 0.2M fraction is newly found potent MBLA. There were some relationships with MBLA and L-PHA but no similarities were observed between MBLA and E-PHA.