생약중의 감초에 관한 연구(I) HPLC에 의한 Glycyrrhizin 및 Glycyrrhetinic acid의 정량

• 영문명: Studies on Licorice in Drug Preparations(I) Determination of Glycyrrhizin and Glycyrrhetinic acid by HPLC.
• 발행기관: 대한약학회
• 저자명: 백남호(Nam Ho Paik) 박만기(Man Ki Park) 박정일(Jeong Hill Park) 김중선(Chung Sun Kim) 서정진(Jung Jin Suh)
• 간행물 정보: 『약학회지』제25권 제1호 (1981년), 1~7쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1981.04.10

영문 초록

Glycyrrhizin (GA) content in licorice was determined by a couple of methods using HPLC, respectively. In Method (I), GA content itself was determined from the licorice aqueous extract, while in Method (II) glycyrrhetinic acid (GHeA ; the aglicone of GA) content corresponding to the quantity of GA was measured from the chloroform extract of the hydrolyzed product of licorice aqueous extract. A reverse phase column Hibar(R) Lichrosorb RP-18 (E. Merck) was used as the stationary phase. As the mobile phase MeOH: H20 (0.05M-NaH2PO4) = 58:42 solution in Method (I), and MeOH: H20: AcOH = 78:19:3 solution in Method (II) were suitable, respectively. The value obtained by Method (II) appeared slightly higher than that by Method (I). The effect of some other herbal drugs on the assay of GA quantity in mixed sample was also observed in both above two methods. By Method (I) Cassiae Cortex, Rehmaniae Rhizoma, Paeoniae Radix, and Angelicae Radix gave the subtractive effect on the amount of GA compared with the value from licorice alone. In the case of Method (II) Cassiae Cortex and Rehmaniae Rhizoma appeared to have subtractive effect but Paeoniae Radix and Angelicae Radix scarcely showed any influence. Pachymae Fungus did not affect the GA content at all. It seems that glycyrrhizin in licorice interacts with certain components of other herbal drugs.