방사선 조사가 쥐의 뇌와 간의 Monoamine Oxidase 활성도에 미치는 영향

• 영문명: Effect of gamma-ray Irradiation on the Activities of Monoamine Oxidase in Rat Brain and Liver
• 발행기관: 대한방사선종양학회
• 저자명: 김주영(Joo Young Kim) 최명선(Myung Sun Choi) 최명언(Myung Un Choi)
• 간행물 정보: 『대한방사선종양학회지』제11권 제2호, 205~217쪽, 전체 13쪽
• 주제분류: 의약학 > 종양학
• 파일형태: PDF
• 발행일자: 1993.12.29

영문 초록

In order to evalute the effects of radiation on mammalian neuronal system, we have examined the effect of gamma-ray radiation on the monoamine oxidase (MAO) activity in monoaminergic neurons. Following the whole body irradiation, MAO activity in the rat brain was measured as well as in the liver for the comparative studies between the neuronal and nonneuronal system. The effects of some radiation protectors and sensitizers were also examined in addition to the O_2 effect. The results can be summarized as follows. 1) The MAO activity of rat brain was minimally affected by the radiation dose up to 1,700 cGy Radiation dose above 2,500 cGy inhibited the brain MAO activity by no less than l0%. MAO-A form was found to be particularly sensitive to radiation. The liver MAO was somewhat inhibited (by about 5%) but hardly dependent on the dose of radiation. 2) The inhibitory effect on the brain was initiated immediately by the radiation dose of 2,500 cGy. On the contrary, for the liver, the inhibitory effect became apparent only 2 days after irradiation. 3) Two days after a dose of 2,500 cGy, Vmax and Km of the brain mitochondrial MAO decreased. For liver, Vmax decreased while Km increased, which indicates the kinetic patterns for the neuronal and nonneruronal systems are not affected similarly by radiation. 4) The effect of several known radiation protectors and sensitizers on MAO activity was tested ut no definite results were obtained. The level of -SH group increased in some degree upon radiation but not by the compounds. 5) MAO activity was not affected by O_2 concentration, while an elevated level of lipid peroxidase was found under the same condition. The results described here indicate that characteristics of MAO, one of the most important central nervous system enzymes, are liable to radiation, which is partially differentiated from the liver MAO. Also indicated are that the -SH groups are hardly related to the effect of radiation but the production of the lipid peroxide seems to be somewhat correlated to the effect of radiation.

키워드

Brain and liver MAO activity Radiation