3,3′,4,4′-tetrachlorobiphenyl (PCB77) enhances human Kv1.3 channel currents and alters cytokine production

• 발행기관: 대한생리학회-대한약리학회
• 저자명: Jong-Hui Kim Soobeen Hwang Seo-In Park Hyo-Ji Lee Yu-Jin Jung Su-Hyun Jo
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제28권 제4호, 323~333쪽, 전체 11쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2024.07.01

국문 초록

Polychlorinated biphenyls (PCBs) were once used throughout various industries; however, because of their persistence in the environment, exposure remains a global threat to the environment and human health. The Kv1.3 and Kv1.5 channels have been implicated in the immunotoxicity and cardiotoxicity of PCBs, respectively. We determined whether 3,3′,4,4′-tetrachlorobiphenyl (PCB77), a dioxin-like PCB, alters human Kv1.3 and Kv1.5 currents using the Xenopus oocyte expression system. Exposure to 10 nM PCB77 for 15 min enhanced the Kv1.3 current by approximately 30.6%, whereas PCB77 did not affect the Kv1.5 current at concentrations up to 10 nM. This increase in the Kv1.3 current was associated with slower activation and inactivation kinetics as well as right-shifting of the steady-state activation curve. Pretreatment with PCB77 significantly suppressed tumor necrosis factor-α and interleukin-10 production in lipopolysaccharide-stimulated Raw264.7 macrophages. Overall, these data suggest that acute exposure to trace concentrations of PCB77 impairs immune function, possibly by enhancing Kv1.3 currents.

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키워드

Cytokines Kv1.3 channel Kv1.5 channel Macrophages PCB77