총백추출물의 고지방식이 유도 비만 마우스에서의 항비만 효과 및 근육조직에서의 에너지대사 조절기전 연구

• 영문명: Anti-Obesity Effects and the Regulation of Energy Metabolism in Skeletal Muscle Tissues of Allii Fistulosi Bulbus Extract in High Fat Diet-Induced Obesity Mice
• 발행기관: 한방비만학회
• 저자명: 최윤용 이현수 백수연 임수민 정효원 강석용 박용기
• 간행물 정보: 『대한한방비만학회지』제22권 제2호, 102~114쪽, 전체 13쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2022.12.31

영문 초록

Objectives: We investigated the effects of Allii Fistulosi Bulbus (AFB) on high fat diet (HFD)-induced obesity in mice and the regulation of energy metabolism in muscle tissues of mice. Methods: The C57BL/6 mice (6 weeks, male) were fed a HFD for 8 weeks and then administrated with AFB extract at 500 mg/kg (p.o.) once daily for 4 weeks. The body weight (BW), muscle weight, calorie intake, fasting blood glucose (FBG) and serum glucose, insulin, and low-density lipoprotein-cholesterol (LDL-C) levels were measured in mice. It was also observed the histological changes of pancreas, liver, and fat tissues with hematoxylin and eosin staining. It was investigated the phosphorylation of insulin receptor substrate 1 (IRS-1), Ser/Thr kinase (AKT), and adenosine monophosphate-activated protein kinase (AMPK), and the expression of phosphoinositide 3-kinase, glucose transporter type 4 (GLUT4), and sirtuin1 (Sirt1) in gastrocnemius tissues by western blot, respectively. Results: The increases of BWs, calorie intakes and FBG levels in obesity mice were decreased significantly by the administration of AFB extract. The AFB extract administration was reduced significantly serum levels of glucose, insulin, and LDL-C in obesity mice. The AFB extract inhibited lipid accumulation in liver tissues, hyperplasia of pancreatic islets, and enlargement of fat tissues in obesity mice. The phosphorylation of IRS-1 and AKT was increased significantly in muscle tissues and AMPK phosphorylation and the GLUT4 and Sirt1 expression were decreased significantly in muscle tissues after the AFB administration. Conclusions: Our study indicates that AFB extract improves symptoms of obesity through regulation of energy regulating proteins in muscle tissues.

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키워드

Obesity High-fat diet Allii Fistulosi Bulbus Energy metabolism