한국의 한 대학병원에서 수집된 Acinetobacter baumannii 임상분리주의 meropenem 내성에 대한 AdeABC 유출펌프의 역할

• 영문명: The role of AdeABC efflux pump to meropenem resistance in Acinetobacter baumannii clinical isolates collected from a university hospital in Korea
• 발행기관: 조선대학교 의학연구원
• 저자명: 심재광(Jae-Kwang Shim) 최지애(Ji Ae Choi) 김춘미(Choon-Mee Kim) 고영진(Young Jin Ko) 강성호(Seong-Ho Kang) 박건(Geon
• 간행물 정보: 『The Medical Journal of Chosun University』제45권 제2호, 75~79쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2020.10.31

영문 초록

The aim of this study is to investigate the role of the AdeABC efflux pump to meropenem resistance in 116 clinical isolates of Acinetobacter baumannii collected from a university hospital in Korea. We performed real-time RT-PCR to test the relative expression of adeB efflux pumps gene in them. The ratio of meropenem MIC change due to carbonyl cyanide m-chlorophenylhydrazone (CCCP), efflux pump inhibitor, was calculated. It was judged that the efflux pump contributed to meropenem resistance when the changed ratio of meropenem MIC by CCCP was 4 or more. The mean and standard error of the adeB relative expression level in the susceptible and resistant groups to meropenem were 3.2±0.8 and 11.9±2.9, respectively and there was a significant difference between the two groups (p＜0.0001). The ratio of meropenem MIC changes due to CCCP to 4 or higher in the susceptible and resistant groups to meropenem were 17.4% and 20.4%, respectively, and there was no significant difference between them. No correlation was observed between the adeB expression and the ratio of meropenem MIC change due to CCCP. It is considered that the adeB efflux pump did not significantly affect meropenem resistance in most strains of A. baumannii tested in this study.

목차

서론

대상 및 방법

결과

고찰

감사의 글

참고문헌

키워드

Acinetobacter baumannii adeB protein Bacterial Drug resistance Meropenem