Docosahexaenoic acid reduces adenosine triphosphate-induced calcium influx via inhibition of store-operated calcium channels and enhances baseline endothelial nitric oxide synthase phosphorylation in human endothelial cells

• 발행기관: 대한생리학회-대한약리학회
• 저자명: Thom Thi Vu Peter Dieterich Thu Thi Vu Andreas Deussen
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제23권 제5호, 345~356쪽, 전체 12쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2019.09.30

영문 초록

Docosahexaenoic acid (DHA), an omega-3-fatty acid, modulates multiple cellular functions. In this study, we addressed the effects of DHA on human umbilical vein endothelial cell calcium transient and endothelial nitric oxide synthase (eNOS) phosphorylation under control and adenosine triphosphate (ATP, 100 M) stimulated conditions. Cells were treated for 48 h with DHA concentrations from 3 to 50 M. Calcium transient was measured using the fluorescent dye Fura-2-AM and eNOS phosphorylation was addressed by western blot. DHA dose-dependently reduced the ATP stimulated Ca2+-transient. This effect was preserved in the presence of BAPTA (10 and 20 M) which chelated the intracellular calcium, but eliminated after withdrawal of extracellular calcium, application of 2-aminoethoxy-diphenylborane (75 M) to inhibit store-operated calcium channel or thapsigargin (2 M) to delete calcium store. In addition, DHA (12 M) increased ser1177/thr495 phosphorylation of eNOS under baseline conditions but had no significant effect on this ratio under conditions of ATP stimulation. In conclusion, DHA dose-dependently inhibited the ATP-induced calcium transient, probably via store-operated calcium channels. Furthermore, DHA changed eNOS phosphorylation suggesting activation of the enzyme. Hence, DHA may shift the regulation of eNOS away from a Ca2+ activated mode to a preferentially controlled phosphorylation mode.

목차

INTRODUCTION

METHODS

RESULTS

DISCUSSION

키워드

Adenosine triphosphate Calcium Docosahexaenoic acid Human umbilical vein endothelial cell Store-operated calcium channels