자운영(Astragalus sinicus L.) 배축절편으로부터 식물체 재생

• 영문명: Plant regeneration from hypocotyls explants of Astragalus sinicus L.
• 발행기관: (사)한국식물생명공학회
• 저자명: 박민선(Min Sun Park) 최필선(Pil Son Choi)
• 간행물 정보: 『Journal of Plant Biotechnology』42권 4호, 396~400쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.12.31

영문 초록

To investigate the optimal conditions for shoot organogenesis in Astragalus sinicus L., hypocotyl explants were cultured in Murashige & Skoog’s (MS) medium supplemented with 0.1, 1.0, 2.0, or 4.0 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) for 6 weeks. 2,4-D concentration significantly effected morphogenesis: some produced calli with adventitious shoots and roots, some produced calli with adventitious roots, some produced only calli, and some produced deep-brownish calli with roots. The formation of calli with shoots and/or roots was observed at lower levels of 2,4-D, whereas calli without shoots or with deep-brownish roots were formed after treatment with higher levels of 2,4-D. Also, a shoot organogenesis ability of callus clones was observed after treatment with medium with 0.1 or 1.0 mg/L 2,4-D grown in MS medium with combinations of benzyl adenine (BA) and 2,4-D for 4 weeks. Medium with a combination of BA and 2,4-D was effective for shoot formation, whereas root organogenesis from calli decreased. The greatest amount of shoot formation was obtained when calli were cultured in MS medium containing 1.0 mg/L 2,4-D and 0.5 mg/L BA. Upon shoot transfer into ½ MS basal medium, plantlets developed, and the plantlets grew well in soil in a greenhouse.

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키워드

Astragalus sinicus Hypocotyl explants Plantlets Shoot organogenesis