일본잎갈나무(Larix kaempferi) 유기질소원 및 식물생장조절물질 처리에 따른 조직증식 및 체세포배 유도 효과

• 영문명: Effect of tissue proliferation and somatic embryo induction in Larix kaempferi following treatment with organic nitrogen sources and plant growth regulators
• 발행기관: (사)한국식물생명공학회
• 저자명: 김용욱(Yong Wook Kim) 김지아(Ji Ah Kim) 문흥규(Heung Kyu Moon) 정수진(Su Jin Jeong)
• 간행물 정보: 『Journal of Plant Biotechnology』42권 4호, 376~379쪽, 전체 4쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.12.31

영문 초록

This study was conducted to evaluate the effects of different types and concentrations of organic nitrogen sources (L-Glutamine and casein hydrolysate, CH) and plant growth regulators (auxins and cytokinins) on embryogenic tissue proliferation and somatic embryo production in L. kaempferi. Overall, the highest tissue fresh weight was obtained at either 2 or 4 weeks in culture when 1,000 mg/L L-Glutamine was added to the culture medium, which showed similar results with other treatments. In experiments with different types and concentrations of plant growth regulators on somatic embryo production, the highest production (426.3/90 mg tissue) was found when 0.2 mg/L IBA was added; however, no somatic embryos were induced following treatment with 0.2 mg/L BA or Kinetin. The effect of various concentrations of IBA on somatic embryo production was also tested. The best result (303/90 mg tissue) was obtained when plants were treated with 0.2 mg/L IBA; 1.0 mg/L IBA was also effective (281/90 mg tissue). The lowest result (109.3/90 mg tissue) was obtained with 5.0 mg/L IBA.

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키워드

Embryogenic tissue IBA Japanese larch Tissue growth