스프레이국화 ‘퍼플엔디’의 꽃잎 배양에 있어서 식물체 재분화요인

• 영문명: Factors influencing shoot regeneration from petal explant in spray mum ‘Purple ND’
• 발행기관: (사)한국식물생명공학회
• 저자명: 이현숙(Hyun Suk Lee) 박현로(Hyun Rho Park) 김현석(Hyun Seak Kim) 김창길(Chang Kil Kim)
• 간행물 정보: 『Journal of Plant Biotechnology』42권 4호, 370~375쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.12.31

영문 초록

This experiment compared the regeneration conditions of the radiation mutant spray chrysanthemum ‘purple ND’. The four different flower blooming stages (S1: 10% opened flower, S2: 30% opened flower, S3: 50% opened flower, and S4: 70% opened flower) and different petal parts (TBOP: the basal of petal and TEOP: the end of petal) were used to compare regeneration conditions between plants grown in MS medium supplemented with IAA and BAP. The highest adventitious shooting rate was identified in plants grown on the IAA 1.0mg·L -1 and BAP 2.0mg·L -1 when using the end of petal at the S2 stage. It displayed 79.2% regeneration and produced 33.4 shoots. Rooted plantlets were successfully established in the greenhouse, showing the same morphological characteristics of vegetative and reproductive organs with those of the mother plant. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions.

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서 론

재료 및 방법

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키워드

Spray chrysanthemum Adventitious shoot Petal explants IAA BAP