현사시나무(Populus alba × P. glandulosa)에서 분리한 non-specific Lipid Transfer Protein (ns-LTP) 프로모터의 특성 분석

• 영문명: Characterization of a non-specific Lipid Transfer Protein (ns-LTP) promoter from poplar (Populus alba × P. glandulosa)
• 발행기관: (사)한국식물생명공학회
• 저자명: 조진성(Jin Seong Cho) 노설아(Seol Ah Noh) 최영임(Young Im Choi)
• 간행물 정보: 『Journal of Plant Biotechnology』42권 4호, 356~363쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.12.31

영문 초록

In order to study genetic engineering in trees, the characterization of genes and promoters from trees is necessary. We isolated the promoter region (867 bp) of Pagns-LTP from poplar (P. alba × P. glandulosa) and characterized its activity in transgenic poplar plants using a β-glucuronidase (GUS) reporter gene. High-level expression of the Pagns-LTP transcript was found in poplar roots, while comparatively low-level expression was found in the young leaves. Pagns-LTP mRNA was not detected in other poplar tissues. Additionally, transgenic poplar plants that contained a Pagns-LTP promoter fused to a GUS reporter gene, displayed tissue-specific GUS enzyme activity localized in root tissue. In silico analysis of the Pagns-LTP promoter sequence reveals the presence of several cis-regulatory elements responsive to phytohormones, biotic and abiotic stresses, as well as those regulating tissue-specific expression. These results demonstrate that the Pagns-LTP promoter has tissue-specific expression activity in poplar roots and leaves that may be involved in organ development and plant resistance to various stresses. Therefore, we anticipate that the Pagns-LTP promoter would be a useful tool to genetically optimize woody plants for functional genomics.

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키워드

Poplar Promoter-GUS fusion Non-specific Lipid Transfer Protein Tissue specific