해양 규조류 Nitzschia sp.의 초저온동결보존을 위한 보존제의 영향 분석

• 영문명: Screening of cryoprotectants (CPAs) for cryopreservation in the Nitzschia sp. of marine microalgae
• 발행기관: (사)한국식물생명공학회
• 저자명: 이인혜(In Hye Lee) 전지영(Ji Young Jeon) 김경미(Kyeung Mi Kim) 강명석(Myung suk Kang)
• 간행물 정보: 『Journal of Plant Biotechnology』45권 4호, 400~408쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.12.31

영문 초록

Biodiversity has continued to degrade in the 21st century due to global warming occasioned by destruction of the environment around the world.. The Nagoya protocol places Korea in a unique position to effectively develop and protect its domestic genetic resources. Microalgae under study in this research contains large amount of antioxidant substances such as beta carotene and astaxanthin, that can be used as biological resource owing to the large amounts of biomass that can be secured through photosynthesis. However, it is difficult to preserve it since cryopreservation method used for long-term preservation is yet to be developed. A basic study for long term cryopreservation was carried out on Nizschia frustulum and Nitzschia amabilis which belong to marine diatoms. As cryoprotectants (CPAs), glycerol, DMSO, and methanol which penetrate into cells were prepared at 5%, 10%, and 15% concentrations each, in case of methanol, it was tested at concentrations of 5%, 10% and 12% by its nature. Two kinds of microalgae, N. frustulum and N. amabilis, were diluted with 102, 103 and 104 cells ml-1, respectively. The highest survival rate was shown at12% concentration of methanol, and the figures were 6.94 ± 0.31% in N. frustulum and 8.85 ± 0.16% in N. amabilis. As a result of 3 weeks cultivation of thawed microalgae after freezing, the result is shows that N. frustulum increased about 10 times faster and N. amabilis increased about 12 times the original concentration.

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키워드

microalgae diatom cryoprotectants(CPAs)