Expression and phosphorylation analysis of soluble proteins and membrane-localised receptor-like kinases from Arabidopsis thaliana in Escherichia coli

• 발행기관: (사)한국식물생명공학회
• 저자명: Eun-Seok Oh Foyjunnaher Eva Sang-Yun Kim Man-Ho Oh
• 간행물 정보: 『Journal of Plant Biotechnology』45권 4호, 315~321쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.12.31

영문 초록

Molecular and functional characterization of proteins and their levels is of great interest in understanding the mechanism of diverse cellular processes. In this study, we report on the convenient Escherichia coli-based protein expression system that allows recombinant of soluble proteins expression and cytosolic domain of membrane-localised kinases, followed by the detection of autophosphorylation activity in protein kinases. This approach is applied to regulatory proteins of Arabidopsis thaliana, including 14-3-3, calmodulin, calcium-dependent protein kinase, TERMINAL FLOWER 1(TFL1), FLOWERING LOCUS T (FT), receptorlike cytoplasmic kinase and cytoplasmic domain of leucine-rich repeat-receptor like kinase proteins. Our Western blot analysis which uses phospho-specific antibodies showed that five putative LRR-RLKs and two putative RLCKs have autophosphorylation activity in vitro on threonine and/or tyrosine residue(s), suggesting their potential role in signal transduction pathways. Our findings were also discussed in the broader context of recombinant expression and biochemical analysis of soluble and membrane-localised receptor kinases in microbial systems.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgments

Conflicts of Interest

Reference

키워드

Calcium-dependent protein kinase Leucine-rich repeat receptor-like kinase (LRR-RLKs) Receptor-like cytoplasmic kinases (RLCKs) Post-translational modification Arabidopsis thaliana Escherichia coli