Optimization of factors influencing in vitro immature seed germination in Chionanthus retusus

• 발행기관: (사)한국식물생명공학회
• 저자명: Khin Yae Kyi Tar Aung Htay Naing Trinh Ngoc Ai Mi Young Chung Chang Kil Kim
• 간행물 정보: 『Journal of Plant Biotechnology』45권 4호, 347~356쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.12.31

영문 초록

Chionanthus retusus is a small deciduous tree that is widely used in landscaping due to its beautiful white spring flowers and ornamental value. Conventional propagation through seeds requires one to two years of breaking dormancy. The objective of this study was to determine the conditions of in vitro germination in C. retusus. In vitro embryo culture was carried out to investigate the effects of six factors: basal media (McCown Woody Plant Medium (WPM) and Murashige and Skoog (MS)); plant growth regulators (different combinations and concentrations of naphthaleneacetic acid (NAA), 6-Benzylaminopurine (BA), and gibberellic acid (GA₃)); embryo age (collected weekly beginning 36 days after fruit setting); low temperature pretreatment (storing 4°C for 1, 2, 3, and 4 weeks); coconut additives (100, 200, and 300 ml·L-1); and genotype (grouping plants depending on their flowering nature). The basal medium used in this study was WPM with 2 mg·L-1-1 GA₃, 20 g·L-1 sucrose, and 6 g·L-1 Agar. WPM medium mixed with GA₃, resulted in higher germination rate as compared to when using a combination of auxin and cytokinin. GA₃ at 2 mg·L-1 was the most effective of all combinations and concentrations of PGRs. WPM medium with 2 mg·L-1 GA₃ resulted in better and faster germination (75.93%). Embryos collected at 57 days after fruit setting had the highest percent of germinated seeds (87.04%) while low-temperature pretreatment of fruits at 4°C for two weeks produced the highest germination (95.37%). These results of this study could be an open ground for development of an efficient protocol for commercial production of the ornamental tree.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

Reference

키워드

Chionanthus retusus embryo culture in vitro germination