Cryopreservation of Hevea brasiliensis zygotic embryos by vitrification and encapsulation-dehydration

• 발행기관: (사)한국식물생명공학회
• 저자명: Korakot Nakkanong Charassri Nualsri
• 간행물 정보: 『Journal of Plant Biotechnology』45권 4호, 333~339쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.12.31

영문 초록

The mature zygotic embryos of the Hevea brasiliensis were cryopreserved through the use of the vitrification and encapsulation/dehydration techniques. In all the experiments, the zygotic embryos were pre-cultured for three days in the MS medium supplemented with 0.3 M sucrose before they were used for the cryopreservation technique. In the vitrification procedure, the effect of the plant vitrification solutions (PVS2 and PVS3) and exposure time were studied. The highest survival rate (88.87%) and regrowth (66.33%) were achieved when the precultured zygotic embryos were incubated in a loading solution for 20 minutes at 0°C. They were subsequently exposed to PVS2 for 120 minutes at 0°C and plunged directly into liquid nitrogen. Cryopreservation by the encapsulation-dehydration method was successfully done by leaving the encapsulated zygotic embryos in a laminar flow for 4 hours prior to plunging into a LN. The survival rate and regrowth of the encapsulated zygotic embryos were 37.50% and 27.98%, respectively. The cryopreserved zygotic embryos were able to develop into whole plants.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

Acknowledgements

Reference

키워드

zygotic embryo Hevea brasiliensis cryopreservation vitrification encapsulation-dehydration