양막상피 제거 유무에 따른 인체 결막상피세포의 증식 속도 비교

• 영문명: Differentiation and Morphological Feature of Conjunctival Epithelial Cells according to Amniotic Membrane Epithelial Cells Removal
• 발행기관: 조선대학교 의학연구원
• 저자명: 김용욱(Long yu Jin) 민지상(Ji Sang Min) 정우진(Woo Jin Jung) 박우찬(Woo Chan Park)
• 간행물 정보: 『The Medical Journal of Chosun University』제43권 제2호, 78~87쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2018.10.31

영문 초록

This study was designed to compare re-epithelization rate/feature of conjunctival epithelial cell on amniotic membrane and anatomical difference between normal amniotic membrane and de-epithelial amniotic membrane. Human conjunctival tissue obtained while cataract operation were used in this study. Primary cultured human conjunctival epithelial cell and fibroblast were incubated on intact amniotic membrane and de-epithelialized amniotic membrane. After incubation for 3, 5 and 7 days, re-epithelization rate was analyzed using cell tracker and microscopic feature was examined using methylene blue and hema-toxylin-eosin stain. For cell marker analysis, cytokeratin 14 and vimentin antibody immunofluorescence stain were used. Clearance rate of epithelial cells on amniotic membrane using trypsin was better than using 25% alcohol. The results of re-epithelization rate using cell tracker, HaCaT, conjunctival firbroblast, and epithelial cell were rapidly incubated on de-epithelialized amniotic membrane. Wound healing and re-epithelization were more rapidly induced on de-epithelialized amniotic membrane on permanent amniotic membrane graft.

목차

서론

대상 및 방법

결과

고찰

키워드

Amniotic membrane epithelium Amniotic membrane transplantation Conjunctival epithelium