학술논문
HPLC-ELSD를 이용한 발효유 제품 중의 Isomaltooligosaccharides 분석법 개발
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- 영문명
- 발행기관
- 한국축산식품학회
- 저자명
- 고진혁 이문석 곽병만 안장혁 박종수 권중호
- 간행물 정보
- 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제33권 제3호, 417~424쪽, 전체 8쪽
- 주제분류
- 농수해양 > 식품과학
- 파일형태
- 발행일자
- 2013.06.30
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국문 초록
영문 초록
A rapid and simple analytical method for the determination of 9 isomaltooligosaccharides (IMO) species in yoghurts was developed using dispersive solid phase extraction (dSPE) clean-up technic and high performance liquid chromatography with evaporative light-scattering detector (HPLC-ELSD). In this study, 9 IMO were extracted from samples simply with chemical reagent using ISO22662 IDF198 method and additional dSPE clean-up. The optimum instrument conditions for the determination were used carbohydrate ES 5μ column with gradient elution of water and acetonitrile and ELS detector. The linearity of this method was expressed as the correlation coefficient (r2), the results of IMO 9 species were shown in 0.9999. LOD and LOQ were respectively 7.9-22.1 mg/kg, 25.9-72.8 mg/kg. The accuracy of intra- and inter day measurements were in the range from 84.3±4.5 to 104.9±6.5%, and the preceision of the intra- and inter-day measurements were in the range from 0.8 to 7.7%. The recoveries were from 84.3±4.5 to 104.9±6.5%. The determination results of IMO 9 species for the 9 yoghurts circulated in the market were in the range from 0.317±0.007 to 1.624±0.050 g/100 g. The newly developed method is appropriate for the determination of IMO in yoghurts, is a rapid and simple method with excellent resolution in compared with previous method.
목차
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