서울지역 식육판매점의 우육에 대한 미생물학적 오염도 평가

• 발행기관: 한국축산식품학회
• 저자명: 고은경 허은정 김영조 박현정 위성환 문진산
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제33권 제3호, 403~410쪽, 전체 8쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2013.06.30

영문 초록

This study was performed to evaluate the microbiological contamination level of raw beef from retail markets in Seoul, Korea. The sampling and laboratory test were performed according to the procedure of “Standard for processing and ingredients specification of livestock product” and “Korean food code”. Enterotoxin of Staphylococcus aureus isolates were detected using VIDAS？ and PCR-based methods. Listeria monocytogenes serotyping and genotyping were carried out using Listeria antisera and L. monocytogenes Fingerprinting kit, respectively. A total of 48 samples were collected from 16 retail markets (butcher`s shop: 5, department store: 6, supermarket: 5) in 2011. The level of total bacteria counts in the butcher`s shop, department store and supermarket were 4.4×103 CFU/g, 3.9×105 CFU/g and 1.0×104 CFU/g, respectively. The concentrations of Escherichia coli of these three retail markets were 6.4×10 CFU/g, 7.6 CFU/g and 2.0×10 CFU/g, respectively. Salmonella species was not detected on all samples. However, S. aureus was isolated in the 3 samples (6.25%) from each type of three retail markets. L. monocytogenes was isolated in the 4 samples (8.3%) from department stores. The level of contamination of these foodborne bacteria was less than 100 CFU/g. The enterotoxin-encoding genes of S. aureus isolates were sea, seh, sei and sep gene. The gene similarity of L. monocytogenes isolated from two retail markets by Rep PCR showed 57.8-98.1% and 68.1-98.1%, respectively. These results suggest that the HACCP guideline for environmental control in slaughterhouse and retail markets should be provided to prevent cross contamination and manage foodborne pathogens such as L. monocytogenes and S. aureus.

키워드

retail markets raw beef HACCP microbiological quality