Simultaneous Determination of Vitamin A and E in Infant Formula by HPLC with Photodiode Array Detection

• 발행기관: 한국축산식품학회
• 저자명: Hong Min Lee Byung Man Kwak Jang Hyuk Ahn Seung Hwan Jeong Sung Lye Shim Kyong Su Kim Taeh Yung Yoon
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제31권 제2호, 191~199쪽, 전체 9쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2011.04.30

영문 초록

The objective of this study was to develop a method to simultaneously quantify vitamins A and E in infant formula. To determine the vitamin A and E content, vitamin A and four different vitamin E isomers (α-, β-, γ-, and δ-tocopherol) were separated by high performance liquid chromatography with a photodiode array detector using a Develosil RPAQUEOUS RP-C30 column (4.6×250 mm, 5 μm). The vitamin A and E contents in the certified reference material determined using this method were within the certified range of standard values. The limits of detection (LODs) and limits of quantitation (LOQs) for vitamin A were 0.02 and 0.06 μg/L, respectively. LODs and LOQs for the vitamin E isomers ranged from 0.20 to 0.55 and from 0.67 to 1.81 μg/L, respectively. Linear analyses indicated that the square of the correlation coefficient for the vitamin A and E isomers was 0.9997-0.9999. The recovery of vitamins ranged from 96.69 to 97.79%. The results demonstrate that this novel method could be used to reliably analyze vitamin A and E content in infant formula.

키워드

vitamin A vitamin E high performance Liquid chromatography photodiode array detector normal phase reverse phase