Medium Optimization for the Production of Probiotic Lactobacillus acidophilus A12 Using Response Surface Methodology

• 발행기관: 한국축산식품학회
• 저자명: Na Kyoung Lee Yeo Lang Park Ga Jin Choe Hyo Ihl Chang Hyun Dong Paik
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제30권 제3호, 359~364쪽, 전체 6쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2010.06.30

영문 초록

Lactobacillus acidophilus A12 was isolated from chicken feces for use as an immunostimulating livestock probiotic. The purpose of this study was to optimize the production of L. acidophilus A12 using response surface methodology (RSM). Initially, the influence of growth medium was studied in terms of carbon sources (glucose, fructose, lactose, glycerol, sucrose, ethanol, and mannitol), nitrogen sources (beef extract, yeast extract, malt extract, and tryptone), and inorganic salts (CaCl2, MgSO4, KH2PO4, (NH4)2SO4, FeSO4, and NaCl). Through one factor-at-a time experiment, lactose, yeast extract, and CaCl2 were determined to be the best sources of carbon, nitrogen, and inorganic salt, respectively. The optimum composition was found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl2. Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced, similar to the predicted value.

키워드

Lactobacillus acidophilus probiotics chicken feces medium optimization response surface methodology