The Relaxant Effect of Propofol on Isolated Rat Intrapulmonary Arteries

• 영문명: The Relaxant Effect of Propofol on Isolated Rat Intrapulmonary Arteries
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Guangyan Zhang Jianxiu Cui Yijing Chen Jue Ma
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제18권 제5호, 377~381쪽, 전체 5쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2014.01.01

영문 초록

Propofol is a widely used anesthetic. Many studies have shown that propofol has direct effects on blood vessels, but the precise mechanism is not fully understood. Secondary intrapulmonary artery rings from male rats were prepared and mounted in a Multi Myograph System. The following constrictors were used to induce contractions in isolated artery rings: high K⁺ solution (60 mmol/L); U46619 solution (100 nmol/L); 5-hydroxytryptamine (5-HT; 3 μmol/L); or phenylephrine (Phe; 1 μmol/L). The relaxation effects of propofol were tested on high K⁺ or U46619 precontracted rings. Propofol also was added to induce relaxation of rings preconstricted by U46619 after pretreatment with the nitric oxide synthase inhibitor N^G-nitro-L-arginine methyl ester (L-NAME). The effects of propofol on Ca²⁺ influx via the L-type Ca²⁺ channels were evaluated by examining contraction-dependent responses to CaCl₂ in the absence or presence of propofol (10 to 300 μmol/L). High K⁺ solution and U46619 induced remarkable contractions of the rings, whereas contractions induced by 5-HT and Phe were weak. Propofol induced dose-dependent relaxation of artery rings precontracted by the high K⁺ solution. Propofol also induced relaxation of rings precontracted by U46619 in an endothelium-independent way. Propofol at different concentrations significantly inhibited the Ca²⁺-induced contractions of pulmonary rings exposed to high K⁺-containing and Ca²⁺-free solution in a dose-dependent manner. Propofol relaxed vessels precon-tracted by the high K⁺ solution and U46619 in an endothelium-independent way. The mechanism for this effect may involve inhibition of calcium influx through voltage-operated calcium channels (VOCCs) and receptor-operated calcium channels (ROCCs).

키워드

Calcium influx Endothelium Propofol Pulmonary artery