Bis is Induced by Oxidative Stress via Activation of HSF1

• 영문명: Bis is Induced by Oxidative Stress via Activation of HSF1
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Hyung Jae Yoo Chang-Nim Im Dong-Ye Youn Hye Hyeon Yun Jeong-Hwa Lee
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제18권 제5호, 403~409쪽, 전체 7쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2014.01.01

영문 초록

The Bis protein is known to be involved in a variety of cellular processes including apoptosis, migration, autophagy as well as protein quality control. Bis expression is induced in response to a number of types of stress, such as heat shock or a proteasome inhibitor via the activation of heat shock factor (HSF)1. We report herein that Bis expression is increased at the transcriptional level in HK-2 kidney tubular cells and A172 glioma cells by exposure to oxidative stress such as H₂O₂ treatment and oxygen-glucose deprivation, respectively. The pretreatment of HK-2 cells with N-acetyl cysteine, suppressed Bis induction. Furthermore, HSF1 silencing attenuated Bis expression that was induced by H₂O₂, accompanied by increase in reactive oxygen species (ROS) accumulation. Using a series of deletion constructs of the bis gene promoter, two putative heat shock elements located in the proximal region of the bis gene promoter were found to be essential for the constitutive expression is as well as the inducible expression of Bis. Taken together, our results indicate that oxidative stress induces Bis expression at the transcriptional levels via activation of HSF1, which might confer an expansion of antioxidant capacity against pro-oxidant milieu. However, the possible role of the other cis-element in the induction of Bis remains to be determined.

키워드

Bis HSF1 Oxidative stress ROS