Polyphenols of Rubus coreanum Inhibit Catecholamine Secretion from the Perfused Adrenal Medulla of SHRs

• 영문명: Polyphenols of Rubus coreanum Inhibit Catecholamine Secretion from the Perfused Adrenal Medulla of SHRs
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Byung-Sik Yu Duck-Mi Na Mi-Young Kang Dong-Yoon Lim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제13권 제6호, 517~526쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2009.01.01

영문 초록

The present study was attempted to investigate whether polyphenolic compounds isolated from wine, which is brewed from Rubus coreanum Miquel (PCRC), may affect the release of catecholamines (CA) from the isolated perfused adrenal medulla of the spontaneously hypertensive rats (SHRs), and to establish its mechanism of action. PCRC (20∼180Ռg/ml) perfused into an adrenal vein for 90 min relatively dose-dependently inhibited the CA secretory responses to ACh (5.32 mM), high K^＋ (56 mM), DMPP (100ՌM) and McN-A-343 (100ՌM). PCRC itself did not affect basal CA secretion (data not shown). Also, in the presence of PCRC (60Ռg/ml), the CA secretory responses to veratridine (a selective Na^＋ channel activator (10ՌM), Bay-K-8644 (a L-type dihydropyridine Ca^2＋ channel activator, 10ՌM), and cyclopiazonic acid (a cytoplasmic Ca^2＋-ATPase inhibitor, 10ՌM) were significantly reduced, respectively. In the simultaneous presence of PCRC (60Ռg/ml) and L-NAME (an inhibitor of NO synthase, 30ՌM), the inhibitory responses of PCRC on the CA secretion evoked by ACh, high K^＋, DMPP, and Bay-K-8644 were considerably recovered to the extent of the corresponding control secretion compared with that of PCRC-treatment alone. The level of NO released from adrenal medulla after the treatment of PCRC (60Ռg/ml) was greatly elevated compared with the corresponding basal level. Taken together, these results demonstrate that PCRC inhibits the CA secretion from the isolated perfused adrenal medulla of the SHRs evoked by stimulation of cholinergic receptors as well as by direct membrane-depolarization. It seems that this inhibitory effect of PCRC is mediated by blocking the influx of calcium and sodium into the adrenal medullary chromaffin cells of the SHRs as well as by inhibition of Ca^2＋ release from the cytoplasmic calcium store at least partly through the increased NO production due to the activation of NO synthase.

키워드

PCRC Catecholamine secretion Adrenal medulla Cholinergic receptors Nitric oxide