Overexpression of AMPKՁ1 Ameliorates Fatty Liver in Hyperlipidemic Diabetic Rats

• 영문명: Overexpression of AMPKՁ1 Ameliorates Fatty Liver in Hyperlipidemic Diabetic Rats
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Eunhui Seo Eun-Jin Park Yeonsoo Joe Soojeong Kang Mi-Sun Kim Sook-Hee Hong Mi-Kyoung Park Duk Kyu Ki
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제13권 제6호, 449~454쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2009.01.01

영문 초록

5 -AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic (Ձ) and two regulatory (Ղ and Ճ) subunits. Two isoforms are known for catalytic subunit (Ձ1, Ձ2) and are encoded by different genes. To assess the metabolic effects of AMPKՁ1, we examined the effects of overexpression of adenoviral-mediated AMPKՁ1 in hyperlipidemic type 2 diabetic rats. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is an established animal model of type 2 diabetes that exhibits chronic and slowly progressive hyperglycemia and hyperlipidemia. Thirty five-week-old overt type 2 diabetic rats (n=10) were administered intravenously with Ad.AMPKՁ1. AMPK activity was measured by phosphorylation of acetyl CoA carboxlyase (ACC). To investigate the changes of gene expression related glucose and lipid metabolism, quantitative real-time PCR was performed with liver tissues. Overexpression of AMPKՁ1 showed that blood glucose concentration was decreased but that glucose tolerance was not completely recovered on 7th day after treatment. Plasma triglyceride concentration was decreased slightly, and hepatic triglyceride content was markedly reduced by decreasing expression of hepatic lipogenic genes. Overexpression of AMPKՁ1 markedly improved hepatic steatosis and it may have effective role for improving hepatic lipid metabolism in hyperlipidemic state.

키워드

AMP-activated protein kinase (AMPK) AMPKՁ 1 catalytic subunit Adenovirus Type 2 diabetes OLETF Fatty liver