Involvement of Caspases and Bcl-2 Family in Nitric Oxide-Induced Apoptosis of Rat PC12 Cells

• 영문명: Involvement of Caspases and Bcl-2 Family in Nitric Oxide-Induced Apoptosis of Rat PC12 Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Yeon-Jin Jeong Ji-Yeon Jung Jin-Ha Lee Jin-Hyoung Cho Guem-Sug Lee Sun-Hun Kim Won-Jae Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제10권 제6호, 329~336쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2006.01.01

영문 초록

This study was aimed to investigate the nitric oxide (NO)-induced cytotoxic mechanism in PC12 cells. Sodium nitroprusside (SNP), an NO donor, decreased the viability of PC12 cells in dose- and time- dependent manners. SNP enhanced the production of reactive oxygen species (ROS), and gave rise to apoptotic morphological changes including cell shrinkage, chromatin condensation, and DNA fragmentation. Expression of Bax was not affected, whereas Bcl-2 was downregulated in SNP-treated PC12 cells. SNP augmented the release of cytochrome c from mitochondria into cytosol and enhanced caspase ⁣8, ⁣9, and ⁣3 activities. SNP upregulated both Fas and Fas-L, which are known to be components of death receptor assembly. These results suggest that NO induces apoptosis of PC12 cells through both mitochondria- and death receptor-mediated pathways mediated by ROS and Bcl-2 family.

키워드

Apoptosis Nitric oxide PC12 cells Bcl-2 family Caspase