Anti-Apoptotic Effect of Rheum undulatum Water Extract in Pancreatic β-cell Line,

• 영문명: Anti-Apoptotic Effect of Rheum undulatum Water Extract in Pancreatic β-cell Line,
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Seo Hyun Yoon Mee Sook Hong Joo Ho Chung Sung Hyun Chung
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제8권 제1호, 51~55쪽, 전체 5쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2004.01.01

영문 초록

Sopungsungi-won has been used as a traditional medicine for diabetes and it has been proved to be a potential remedy for type 2 diabetes mellitus. We previously reported that water extract of Sopungsungi-won exhibits anti-diabetic effects both in vivo and in vitro experiments. In the present study, we have chosen to examined anti-apoptotic effect of Rheum undulatum, which is the main component of Sopungsungi-won, on pancreatic β-cells, HIT-T15, against hydrogen peroxide (H₂O₂). oxidative stress. To investigate the anti-apoptotic effect of Rheum undulatum water extract (RUWE) against H₂O₂-induced apoptosis in pancreatic β-cell line of hamster, HIT-T15, MTT assay, DAPI staining, TUNEL assay, RT-PCR and caspase-3 enzyme assay were performed. The morphological analysis demonstrated that cells treated with H₂O₂ exhibited classical apoptotic features, while such changes was reduced in cells pre-treated with RUWE. In addition, RUWE pre-treated cells prior to H₂O₂ treatment induced increase of levels of bcl-2 expression and decrease of caspase-3 enzyme activity compared to cells treated with H₂O₂ only. These results provide the possibility of usage of RU in patients with progressively deteriorated diabetes.

키워드

HIT-T15 Apoptosis Hydrogen peroxide Rheum undulatum water extract Oxidative