Increased Expression of Nitric Oxide Synthases in Left and Right Ventricular Hypertrophy

• 영문명: Increased Expression of Nitric Oxide Synthases in Left and Right Ventricular Hypertrophy
• 발행기관: 대한생리학회-대한약리학회
• 저자명: JongUn Lee Yoon Wha Oh Sunmi Kim Dae Gill Kang Won Jung Lee
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제5권 제1호, 65~70쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2001.01.01

영문 초록

The present study was aimed to explore pathophysiological implications of nitric oxide in the development of left and right ventricular hypertrophy. To induce selective left and right ventricular hypertrophy, rats were made two-kidney, one clip (2K1C) hypertensive and treated with monocrotaline (MCT), respectively. Six weeks later, the hearts were taken and their ventricular tissue mRNA and protein expression of endothelial constitutive isoform of nitric oxide synthase (NOS) were determined by reverse transcription-polymerase chain reaction and Western blot analysis, respectively. In 2K1C hypertensive rats, the expression of NOS mRNA was increased in parallel with its proteins in the left ventricle, but not in the right ventricle. In MCT-treated rats, the expression of NOS mRNA and proteins were proportionally increased in the right ventricle, but not in the left ventricle. These results suggest that the expression of NOS is specifically increased in association with the ventricular hypertrophy, which may be a mechanism counteracting the hypertrophy.

키워드

Heart hypertrophy Nitric oxide synthase ANOVA analysis of variance DTT dithiothreitol Grp glucose-regulated protein HSP heat shock family of stress proteins Hsp heat shock protein PDBu phorbol 12,13-dibutyrate PKC protein kinase C RLC