Effects of Ginsenosides on the Mechanism of Histamine Release in the Guinea Pig Lung Mast Cells Activated by Specific Antigen- Antibody Reactions

• 영문명: Effects of Ginsenosides on the Mechanism of Histamine Release in the Guinea Pig Lung Mast Cells Activated by Specific Antigen- Antibody Reactions
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Jai Youl Ro Young Soo Ahn Kyung Hwan Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제4호, 445~456쪽, 전체 12쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

We previously reported that some components of ginsenosides decreased mediator releases evoked by the activation of mast cells with specific antigen-antibody reactions. This study aimed to assess the effects of ginsenosides (Rb₂, Re) on the mechanism of histamine release in the mast cell activation. We partially purified guinea pig lung mast cells by using enzyme digestion, the rough and the discontinuous percoll density gradient method. Mast cells were sensitized with IgG₁ and challenged with ovalbumin (OA). Histamine was assayed by fluorometric analyzer, leukotrienes by radioimmunoassay. Phospholipase D (PLD) activity was assessed more directly by the production of [³H]phosphatidylbutanol (PBut) which was produced by PLD-mediated transphosphatidylation in the presence of butanol. The amount of 1,2- diacylglycerol (DAG) were measured by the [³H]DAG labeled with [³H]palmitic acid or [³H]myristic acid. Pretreatment of Rb₂ (300 ㄍg) significantly decreased histamine release by 60%, but Re (300 ㄍg) increased histamine release by 34%. Leukotrienes release in Rb₂ was decreased by 40%, Re was not affected in the leukotrienes release during mast cell activations. An increasing PLD activity during mast cell activation was decreased by the dose-dependent manner in the pretreatment of Rb₂, but Re pretreatment facilitated the increased PLD activity during mast cell activation. The amount of DAG produced by phospholipase C (PLC) activity was decreased by Rb₂ pretreatment, but Re pretreatment was not affected. The amount of mass DAG was decreased by Rb₂ and Re pretreatment during mast cell activation. The data suggest that Rb₂ purified from Korean Red Ginseng Radix inhibits the DAG which is produced by the activation of mast cells with antigen-antibody reactions via both phosphatidylinositide-PLC and phosphatidylcholine- PLD systems, and then followed by the inhibition of histamine release. However, Re increases histamine release by stimulation of DAG production, which is mediated by phosphatidylcholine-PLD system rather than by phosphatidylinositide-PLC system, but inhibits the mass DAG production. Thus, it could be inferred that other mechanisms play a role in the increase of histamine release during mast cell activation.

키워드

Mast cell Histamine Leukotrienes Phospholipase D Phospholipase C 1,2- diacylglycerol Phosphatidylbutanol