Distributional Patterns of Phospholipase C Isozymes in Heart and Brain of Spontaneously Hypertensive and Normotensive Rats

• 영문명: Distributional Patterns of Phospholipase C Isozymes in Heart and Brain of Spontaneously Hypertensive and Normotensive Rats
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Ji-Woong Choi Young-Jin Cho Seok Ho Cha Kweon-Haeng Lee Sang-Bok Lee
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제4호, 385~392쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

The phospholipase C (PLC)-mediated intracellular signal transduction pathway is considered to be involved in the regulation of blood pressure. However, little information is available concerning the distributional and functional significance of PLC in the genetic hypertensive rats. As the first step of knowing the role of PLC on hypertension, we investigated the distribution of 6 PLC isozymes (PLC-β1, -β3, -β4, -γ1, -γ2 and -δ1) in the heart and brain, which are concerned with hypertension, in the normotensive Wistar-Kyoto rat (WKY) and spontaneously hypertensive rat (SHR) using the western blotting and immunocytochemistry. The immunoreactivities of PLC isozymes in brain were detected, but there were no distributional and quantitative differences between the WKY and SHR. In the heart, but the immunoreactivities to PLC-β1 and -γ2 in the SHR were higher than those in WKY. In immunocytochemistry to confirm these western blotting data, PLC-β1 and -γ2 were localized in cardiac myocytes and the intensities of immunoreactivity in SHR were stronger than that in WKY. These results suggest that PLC-β1 and -γ2 would have possibility to concern with the establishment of spontaneous hypertension.

키워드

PLC isozymes Western blot Immunocytochemistry Heart Brain SHR