Effect of t-butylhydroperoxide on Na+-dependent Glutamate Uptake in Rabbit Brain Synaptosome

• 영문명: Effect of t-butylhydroperoxide on Na⁺-dependent Glutamate Uptake in Rabbit Brain Synaptosome
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Hyun Je Lee Yong Keun Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제4호, 367~376쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

The effect of an organic peroxide, t-butylhydroperoxide (t-BHP), on glutamate uptake was studied in synaptosomes prepared from cerebral cortex. t-BHP inhibited the Na⁺-dependent glutamate uptake with no change in the Na⁺-independent uptake. This effect of t-BHP was not altered by addition of Ca²⁺ channel blockers (verapamil, diltiazem and nifedipine) or PLA₂ inhibitors (dibucaine, butacaine and quinacrine). However, the effect was prevented by iron chelators (deferoxamine and phenanthroline) and phenolic antioxidants (N,N -diphenyl-phenylenediamine, butylated hydroxyanisole, and butylated hydroxytoluene). At low concentrations (＜1.0 mM), t-BHP inhibited glutamate uptake without altering lipid peroxidation. Moreover, a large increase in lipid peroxidation by ascorbate/Fe²⁺ was not accompanied by an inhibition of glutamate uptake. The impairment of glutamate uptake by t-BHP was not intimately related to the change in Na⁺-K⁺-ATPase activity. These results suggest that inhibition of glutamate uptake by t-BHP is not totally mediated by peroxidation of membrane lipid, but is associated with direct interactions of glutamate transport proteins with t-BHP metabolites. The Ca²⁺ influx through Ca²⁺ channel or PLA₂ activation may not be involved in the t-BHP inhibition of glutamate transport.

키워드

t-butylhydroperoxide Glutamate uptake Lipid peroxidation Na⁺-K⁺-ATPase activity Rabbit brain synaptosomes