Production and Characterization of Specific Antibodies to Bombesin

• 영문명: Production and Characterization of Specific Antibodies to Bombesin
• 발행기관: 대한생리학회
• 저자명: Kwon, Hyeok-Yil Lee, Yun-Lyul Park, Hyoung-Jin
• 간행물 정보: 『대한생리학회지』제28권 제1호, 91~98쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1994.06.30

영문 초록

In order to produce a specific bombesin antiserum far very sensitive radioimmunoassay, synthetic [lys³]-bombesin conjugated to bovine serum albumin was subcutaneously injected into guinea pigs. The conjugation was performed using either carbodiimide or gIutaraldehyde as a coupling agent. The antisera were characterized by analysis of Scatchard and Sips plots. The antiserum LBE 2G/2 raised by repeat injection of the immunogen conjugated with carbodiimide showed the titer of 1 : 188,000, very low cross-reactivity to bombesin-like peptides except bombesin, with high affinity constant (1.64 X 10¹¹ M^-1) and high heterogeneity index (0.91). The antiserum LBG 1G/2 produced by repeat injection of the immunogen conjugated with glutaraldehyde possessed the titer of 1 : 43,000, high cross-reactivity to some bombesin-like peptides, high affinity constant (1.19 X 10¹¹ M^-1) and high heterogeneity index (0.79). These results indicate that the antiserum LBE 2G/2 is specific only to bombesin and that the antiserum LBG IG/2 binds to some bombesin-like peptides such as alytesin, gastrin releasing peptide and neuromedin C. The antiserum LBE 2G/2 is sufficient for the very sensitive radioimmunoassay of bombesin.

키워드

Bombesin antibody Immunization technique Radioimmunoassay