고양이 위(胃)의 수축 및 전기활동에 대한 에탄올과 칼슘의 관계

• 영문명: Relation of Ethanol and Calcium to Contractile and Electrical Activity of Cat Stomach
• 발행기관: 대한생리학회
• 저자명: 김명석(Kim, Myung-Suk) 심상수(Sim, Sang-Soo) 윤신희(Yoon, Shin-Hee) 한상준(Han, Sang-Jun) 김정진(Kim, Chung-Chin) 최
• 간행물 정보: 『대한생리학회지』제21권 제2호, 259~272쪽, 전체 14쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1987.12.30

영문 초록

This was study carried out to investigate the effect of calcium on spontaneous contraction and electrical activity induced by ethanol in gastric smooth muscle. After peeling off the mucous membrane from the isolated whole stomach of 102 cats, two kinds of small muscle preparations (2.0 X 0.2 cm), one longitudinal and the other circular, were excised from the fundus, the corpus and the antrum portion of each whole stomach specimen. The isometric contraction of the small muscle preparation was measured in a cylinder-shaped chamber filled with Krebs-Ringer-dextrose solution (pH 7.4, temperature 36±0.5℃) bubbling with 5% CO₂ in O₂. A large muscle preparation (5.0 X 1.2 cm) was excised from the anterior wall of the corpus-antrum portion of the same specimen in 72 of 102 cats. The gastric electrical activity (slow wave and spike potential) was monopolarly recorded by four capillary electrodes (Ag-AgCl), of which two were placed on the corpus and two on the antrum, in a muscle chamber filled with the same solution as described above. Changes in the amplitude of the contraction, frequency of the gastric slow wave and the production of the spike potential were observed after adding ethanol and/or under the treatments with verapamil, CaCl₂ and Ca-free Krebs-Ringer-dextrose solution. The results were as follows: 1) After adding ethanol, the spontaneous phasic contraction of the corpus was reduced dose-dependently (0.125-2.0%), which was totally abolished by higher concentrations (2.0-8.0%) of ethanol. 2) The corporal phasic contraction was also completely abolished by verapamil (3 X 10^-5 M) or Ca-free Krebs-Ringer-dextrose solution. The contraction was increased by CaCl₂ (1.8 X 10^-3 M), but the inhibitory effect of ethanol on the contraction persisted even under the treatment with CaCl₂. 3) At higher concentrations, ethanol caused tonic contraction of both preparations from the fundus, the corpus and the antrum in a dose-dependent manner. The tonic contraction of the fundus produced by ethanol was not influenced by CaCl₂ or verapamil, whereas the tonic contraction was not produced by ethanol in tile Ca-free solution. 4) Frequency of gastric slow wave was decreased dose-dependently by the addition of ethanol (0.25-1.0%), and tile slow wave was not produced by higher concentration of ethanol (2.0%). 5) The frequency of slow wave was significantly reduced by verapamil only and the inhibitory influence of ethanol on the slow wave frequency was reinforced by verapamil. 6) The treatment of CaCl₂ increased significantly the slow wave frequency, and attenuated the inhibitory effect of ethanol on the frequency. It is therefore suggested that ethanol regulates the phasic contraction and the production of slow wave by interfering with the transport of calcium in the stomach muscle of the cat.

키워드

Stomach Smooth muscle contraction Slow wave Ethanol Calcium Verapamil