학술논문
白鼠 大腦組織의 Alkaline Ribonuclease 의 部分精製 및 特性에 關한 硏究
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- 영문명
- PARTIAL PURIFICATION RIBONUCLEASE FROM AND CHARACTERIZATION OF AIKALINE GEREBRAL TISSUES OF ALBINO RATS
- 발행기관
- 대한신경정신의학회
- 저자명
- 張換一
- 간행물 정보
- 『신경정신의학』제15권 제3호, 208~221쪽, 전체 14쪽
- 주제분류
- 의약학 > 정신과학
- 파일형태
- 발행일자
- 1976.07.30
4,480원
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국문 초록
영문 초록
The alkaline RNase was partially purified from the cerebral tissues of albino rats and its characteristics
influenced by numerous ions, changes of temperature, sulfhydryl reagent and several kinds of surfactants
were observed.
The following results were obtained.
1) The alkaline RNase was about 1200-fold purified by the consecutive processes of heating, salting-
out and DEAE-Cellulose Column Chromatography.
2) The Km value of alkaline RNase, treated with the substrate of repurified yeast RNA, was
568/ig/ml. and followed the kinetics of Michaelis- Menten.
3) The optimal pH of alkaline RNase was 7.8 and the activities of alkaline RNase were maintained
in a relatively broad range of pH.
4) The alkaline RNase was resistant to heat,especially more resistant in a homogenate and was
stable in the presence of the substrates or other proteins.
5) The alkaline RNase was not significantly influenced by low concentration of pHMB (parahydroxy
mercuribenzoate).
6) The activities of alkaline RNase were inhibited by divalent cations and slightly accelerated
by Na+ and K+ in the range of 0. IM. and inhibited
markedly by Li+.
7 ) Sodium dodecyl sulfate inhibited the activities of alkaline RNase markedly, but Tween-80 and
Cetyltrimethylammouium chloride not change its activities.
목차
緒 論
黄驗 方法
實驗結果
考 察
結 論
參 考 文 戴
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