Improvement of Lyophilized Probiotics Viability Using a Histidine-based Rehydration Formula

• 영문명: Improvement of Lyophilized Probiotics Viability Using a Histidine-based Rehydration Formula
• 발행기관: 한국유산균프로바이오틱스학회
• 저자명: 이규연(Kyuyeon Lee) 김나연(Nayeon Kim) 김보배(Bobae Kim) 김혜신(Hyeshin Kim) 한경준(Kyoungjun Han) 여지은(Jieun Yeu) Wilhelm H. Holzapfel 강혜지(Hyeji Kang)
• 간행물 정보: 『Current Topics in Lactic Acid Bacteria and Probiotics』10권 1호, 16~26쪽, 전체 11쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2024.06.30

국문 초록

Bacteria get stressed and damaged during freeze-drying process for commercialization and this result in loss of its effect. Viability is important for its efficacy, but this drying process can deteriorate viability by damaging the integrity of the cell membrane as well. In this study, we propose 0.03 M histidine for rehydration of freeze-dried probiotics to improve their viability. The freeze-dried bacteria mixture with 0.03 M histidine showed augmented survivability during in vitro simulated gastric and duodenum stress conditions and increased viability during 60 min rehydration. It exhibited a significantly increased adherence ability of lyophilized bacteria to the HT-29 cell-line. Therefore, this shows possibility of probiotics commercialization with damage of lyophilization restored and survivability ameliorated.

목차

Introduction

Materials and Methods

Results

Discussion

References

키워드

lactic acid bacteria (LAB) zeta-potential simulated stomach-duodenum passage (SSDP) cell adhesion cell stability