Establishment of Evaluation Method for Bhas 42 Cell Transformation Assay

• 영문명: Establishment of Evaluation Method for Bhas 42 Cell Transformation Assay
• 발행기관: 한국동물보건학회
• 저자명: Jeonghwa Kim Daseul Lee Ohryun Kwon Suyoun Jeong Taewon Kim
• 간행물 정보: 『한국동물보건학회지』3권 2호, 85~95쪽, 전체 11쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2024.06.30

국문 초록

The ICH S1B (R1) appendix has been recently revised to supplement the overall S1 guidelines and used in close conjunction with ICH S1A, S1B, and S1C. This appendix is intended to provide an integrated approach to provide a specific weight of evidence (WoE) for determining whether a 2-year rat carcinogenicity study provides value in performing a human carcinogenic risk assessment. The OECD tried to allow to use the described method as part of a weight of evidence approach in the testing of substances for carcinogenic potential, and proposed a cell transformation assay using Bhas 42 cells. Cell transformation assays were evaluated using Bhas42 cells, and the number of foci with malignant transformation was counted. Transformed foci appeared in the positive material treated group, and it was confirmed that there was a statistically significant increase in the positive material treated group compared to the solvent control group. Before animal experiments for carcinogenicity detection, data from the Bhas 42 cell transformation assay may be considered, if available. In addition, cell transformation assay can morphologically confirm the carcinogenic effects at the initiation.

목차

1. Introduction

2. Materials and Methods

3. Results

4. Discussion

Acknowledgements

Conflict of interests

Orcid

References

키워드

Bhas 42 cell Carcinogenicity Cell transformation assay OECD Weight of evidence