학술논문
Study on the Molecular Mechanism of Antioxidant Health Effect of Tuna Dark Meat Enzymatic Polypeptides
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- 영문명
- Study on the Molecular Mechanism of Antioxidant Health Effect of Tuna Dark Meat Enzymatic Polypeptides
- 발행기관
- East Asian Health Association
- 저자명
- Diya Zhang
- 간행물 정보
- 『Journal of East Asian Health』Vol.1 No.1, 49~56쪽, 전체 8쪽
- 주제분류
- 의약학 > 기타의약학
- 파일형태
- 발행일자
- 2024.03.31
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국문 초록
Purpose – The purpose of this paper is to explore the molecular mechanism of polypeptide antioxidant health, and
effectively promote the new direction of human health functions.
Design/Methodology/Approach – Based on methodology, the paper studies this article uses trypsin to enzymatically
hydrolyze tuna dark meat, obtained an enzymatic biological active polypeptide, and performs peptide sequencing
using matrix-assisted laser dissociation time-of-flight mass spectrometry (MALDI TOF/TOF),obtained the first
-level (MS) and second structure (MS/MS) of the peptide. Finally, the molecular docking was performed through
Discovery Studio (DS), target protein prediction is performed to determine the connection with antioxidant health
care.
Findings – The results show that the dominant peptide sequences in the tuna dark meat trypsin hydrolyzed
polypeptides are SSAR, VSSK and GEHAE; through molecular simulation methods (Discover Studio, DS), the
potential target protein screening of the above enzyme polypeptide is found to be closely linked to Keap1 (oxidation)
protein, so it is determined to have a certain connection with antioxidant molecules.
Research Implications – In the management of this paper, In this article, this study is of great significance in
antioxidant molecules. It provides a new direction for the removal of free radicals and effectively suppress the aging
process. The health effect is significant, which will also provide a reference for the subsequent research of other
scholars.
영문 초록
목차
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