학술논문
Osteoblastogenesis and osteolysis in the Zucker Diabetic Sprague Dawley rat humerus head
이용수 0
- 영문명
- Osteoblastogenesis and osteolysis in the Zucker Diabetic Sprague Dawley rat humerus head
- 발행기관
- 대한해부학회
- 저자명
- Gcwalisile Frances Dlamini Robert Ndou
- 간행물 정보
- 『Anatomy and Cell Biology』Vol.56(4), 552~561쪽, 전체 10쪽
- 주제분류
- 의약학 > 의학일반
- 파일형태
- 발행일자
- 2023.12.31
4,000원
구매일시로부터 72시간 이내에 다운로드 가능합니다.
이 학술논문 정보는 (주)교보문고와 각 발행기관 사이에 저작물 이용 계약이 체결된 것으로, 교보문고를 통해 제공되고 있습니다.
국문 초록
영문 초록
The endocrinology of type 2 diabetes (T2D) and its predisposing factors have been studied extensively while its skeletal effects have received negligible research despite this being a global disease. The cellular and molecular association between proximal humeral fractures and T2D has not been fully elucidated. We aimed to study bone cell quantities and immunolabel osteogenic and antiosteogenic cytokines. The study used 12-week-old rats (23 males) consisting of 8 Sprague Dawley (SD) and 15 Zucker Diabetic Sprague Dawley (ZDSD). Weekly mass measurements were taken while fasting blood glucose levels were recorded every 2 weeks with oral glucose tolerance tests conducted once every 4 weeks. Upon termination at the age of 28 weeks, humeri were fixed in 10% buffered formalin, prior to decalcification in ethylenediaminetetraacetic acid. The bone samples were then processed in ascending grades of alcohol using an automatic processor before embedding in paraffin wax. Sections were cut at 5 μm thickness in a series for Haematoxylin and Eosin stain, and immunohistochemistry was performed with the anti-tartrate-resistant acid phosphatase (TRAP), anti-alkaline phosphatase (ALP), anti-bone morphogenetic protein 3 (BMP3), anti-transforming growth factor beta 1 (TGFβ1), anti-aged glycation end product (AGE) antibodies in the sequence. ZDSD rats had more adipocytes, BMP3 and AGEs expression with higher numbers of TRAP positive osteocytes and fewer ALP cells although no differences were found in TGFβ1 immunopositivity. We also found that T2D increases the number of AGEs immuno-positive cells, as well as its extracellular expression, thus providing a conducive environment for the interaction of the osteogenic cytokine and its antagonist to suppress osteoblastogenesis. ZDSD groups had higher adipocyte numbers therefore increased marrow adiposity in T2D.
목차
Introduction
Materials and Methods
Results
Discussion
ORCID
Author Contributions
Conflicts of Interest
Funding
Acknowledgements
References
해당간행물 수록 논문
- A clinical perspective on the anatomical study of digastric muscle
- Polymelia (thoracomelia), an extremely rare appearance of congenital anomalic limb in a Nepalese child and its embryological basis
- A marginal branch of the left hepatic artery running along the umbilical vein and supplying the anterior surface of the liver left lobe: a report of 5 cases in 12 Japanese human fetuses
- Azygos anterior cerebral artery associated with hypoplastic A1 fragment of right anterior cerebral artery
- Uncommon configuration of intercostobrachial nerves, lateral roots, and absent medial cutaneous nerve of arm in a cadaveric study
- Fenestrated popliteal vein pierced by a branch of the tibial nerve
- Anatomical variation of quadratus plantae in relation with flexor digitorum brevis
- Osteoblastogenesis and osteolysis in the Zucker Diabetic Sprague Dawley rat humerus head
- A comparative study on the hepatoprotective effect of selenium-nanoparticles and dates flesh extract on carbon tetrachloride induced liver damage in albino rats
- Effect of Sofosbuvir on rats’ ovaries and the possible protective role of vitamin E: biochemical and immunohistochemical study
- The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients
- Evaluating the effect of conditioned medium from mesenchymal stem cells on differentiation of rat spermatogonial stem cells
- Rubus fruticosus leaf extract inhibits vascular dementia-induced memory impairment and neuronal loss by attenuating neuroinflammation
- Morphological types and morphometrical measurements of the suprascapular notch in both dry bones and human cadavers: anatomical study to improve the outcomes of the diagnostic and interventional procedures in the shoulder region
- Dental characteristics on panoramic radiographs as parameters for non-invasive age estimation: a pilot study
- Magnetic resonance angiography in assessment of anomalies of anterior cerebral artery in adults
- Histology and immunohistochemistry of the human carotid sinus nerve
- The use of precontoured plates for midshaft clavicle fractures is not always the best course of treatment
- Variations of azygos vein: a cadaveric study with clinical relevance
- Trabeculae in the basilar venous plexus: anatomical and histological study with application to intravascular procedures
- Morphological and morphometric study of pulmonary vein anatomy in relation to cardiac invasive and electrophysiological procedures
- Analyzing the factors that contribute to the development of embryological classical type of bladder exstrophy
- Anatomical variations of the tibial nerve and their clinical correlation
- Botulinum neurotoxin injection for treating plunged nose and post-rhinoplasty: anatomical perspectives of depressor septi nasi, nasalis, leveator labii superioris alaeque nasi muscle
참고문헌
관련논문
의약학 > 의학일반분야 BEST
- 교육전담간호사가 주도하는 임상현장교육 프로그램이 신규 간호사의 간호업무성과와 교육 만족도에 미치는 효과
- 일반인을 위한 해부학 만화
- 중환자실 간호사의 간호근무환경과 직무 스트레스가 간호업무성과에 미치는 영향
의약학 > 의학일반분야 NEW
- 안구내용적출 시 자가통증조절장치를 이용한 선제적 전신 진통제의 효과
- 빅데이터를 이용한 COVID-19 백신 접종 후 발생한 안과적 이상사례에 대한 고찰
- 만성 중심장액맥락망막병증에서 국소레이저광응고술과 유리체 내 베바시주맙 주입술의 효과 비교
최근 이용한 논문
교보eBook 첫 방문을 환영 합니다!
신규가입 혜택 지급이 완료 되었습니다.
바로 사용 가능한 교보e캐시 1,000원 (유효기간 7일)
지금 바로 교보eBook의 다양한 콘텐츠를 이용해 보세요!