학술논문
Evaluating the effect of conditioned medium from mesenchymal stem cells on differentiation of rat spermatogonial stem cells
이용수 0
- 영문명
- Evaluating the effect of conditioned medium from mesenchymal stem cells on differentiation of rat spermatogonial stem cells
- 발행기관
- 대한해부학회
- 저자명
- Hoda Fazaeli Mohsen Sheykhhasan Naser Kalhor Faezeh Davoodi Asl Mojdeh Hosseinpoor Kashani Azar Sheikholeslami
- 간행물 정보
- 『Anatomy and Cell Biology』Vol.56(4), 508~517쪽, 전체 10쪽
- 주제분류
- 의약학 > 의학일반
- 파일형태
- 발행일자
- 2023.12.31
4,000원
구매일시로부터 72시간 이내에 다운로드 가능합니다.
이 학술논문 정보는 (주)교보문고와 각 발행기관 사이에 저작물 이용 계약이 체결된 것으로, 교보문고를 통해 제공되고 있습니다.
국문 초록
영문 초록
In cancer patients, chemo/radio therapy may cause infertility by damaging the spermatogenesis affecting the selfrenewal and differentiation of spermatogonial stem cells (SSCs). In vitro differentiation of stem cells especially mesenchymal stem cells (MSCs) into germ cells has recently been proposed as a new strategy for infertility treatment. The aim of this study was to evaluate the proliferation and differentiation of SSCs using their co-culture with Sertoli cells and conditioned medium (CM) from adipose tissue-derived MSCs (AD-MSCs). Testicular tissues were separated from 2-7 days old neonate Wistar Rats and after mechanical and enzymatic digestion, the SSCs and Sertoli cells were isolated and cultured in Dulbecco’s modified eagle medium with 10% fetal bovine serum, 1X antibiotic, basic fibroblast growth factor, and glial cell linederived neurotrophic factor. The cells were treated with the CM from AD-MSCs for 12 days and then the expression level of differentiation-related genes were measured. Also, the expression level of two major spermatogenic markers of DAZL and DDX4 was calculated. Scp3, Dazl, and Prm1 were significantly increased after treatment compared to the control group, whereas no significant difference was observed in Stra8 expression. The immunocytochemistry images showed that DAZL and DDX4 were positive in experimental group comparing with control. Also, western blotting revealed that both DAZL and DDX4 had higher expression in the treated group than the control group, however, no significant difference was observed. In this study, we concluded that the CM obtained from AD-MSCs can be considered as a suitable biological material to induce the differentiation in SSCs.
목차
Introduction
Materials and Methods
Results
Discussion
ORCID
Author Contributions
Conflicts of Interest
Funding
References
해당간행물 수록 논문
- A clinical perspective on the anatomical study of digastric muscle
- Polymelia (thoracomelia), an extremely rare appearance of congenital anomalic limb in a Nepalese child and its embryological basis
- A marginal branch of the left hepatic artery running along the umbilical vein and supplying the anterior surface of the liver left lobe: a report of 5 cases in 12 Japanese human fetuses
- Azygos anterior cerebral artery associated with hypoplastic A1 fragment of right anterior cerebral artery
- Uncommon configuration of intercostobrachial nerves, lateral roots, and absent medial cutaneous nerve of arm in a cadaveric study
- Fenestrated popliteal vein pierced by a branch of the tibial nerve
- Anatomical variation of quadratus plantae in relation with flexor digitorum brevis
- Osteoblastogenesis and osteolysis in the Zucker Diabetic Sprague Dawley rat humerus head
- A comparative study on the hepatoprotective effect of selenium-nanoparticles and dates flesh extract on carbon tetrachloride induced liver damage in albino rats
- Effect of Sofosbuvir on rats’ ovaries and the possible protective role of vitamin E: biochemical and immunohistochemical study
- The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients
- Evaluating the effect of conditioned medium from mesenchymal stem cells on differentiation of rat spermatogonial stem cells
- Rubus fruticosus leaf extract inhibits vascular dementia-induced memory impairment and neuronal loss by attenuating neuroinflammation
- Morphological types and morphometrical measurements of the suprascapular notch in both dry bones and human cadavers: anatomical study to improve the outcomes of the diagnostic and interventional procedures in the shoulder region
- Dental characteristics on panoramic radiographs as parameters for non-invasive age estimation: a pilot study
- Magnetic resonance angiography in assessment of anomalies of anterior cerebral artery in adults
- Histology and immunohistochemistry of the human carotid sinus nerve
- The use of precontoured plates for midshaft clavicle fractures is not always the best course of treatment
- Variations of azygos vein: a cadaveric study with clinical relevance
- Trabeculae in the basilar venous plexus: anatomical and histological study with application to intravascular procedures
- Morphological and morphometric study of pulmonary vein anatomy in relation to cardiac invasive and electrophysiological procedures
- Analyzing the factors that contribute to the development of embryological classical type of bladder exstrophy
- Anatomical variations of the tibial nerve and their clinical correlation
- Botulinum neurotoxin injection for treating plunged nose and post-rhinoplasty: anatomical perspectives of depressor septi nasi, nasalis, leveator labii superioris alaeque nasi muscle
참고문헌
관련논문
의약학 > 의학일반분야 BEST
- 교육전담간호사가 주도하는 임상현장교육 프로그램이 신규 간호사의 간호업무성과와 교육 만족도에 미치는 효과
- 일반인을 위한 해부학 만화
- 간호대학생의 MBTI 성격유형별 의사소통능력, 자아존중감, 학업성취도의 관계
의약학 > 의학일반분야 NEW
- 안구내용적출 시 자가통증조절장치를 이용한 선제적 전신 진통제의 효과
- 빅데이터를 이용한 COVID-19 백신 접종 후 발생한 안과적 이상사례에 대한 고찰
- 만성 중심장액맥락망막병증에서 국소레이저광응고술과 유리체 내 베바시주맙 주입술의 효과 비교
최근 이용한 논문
교보eBook 첫 방문을 환영 합니다!
신규가입 혜택 지급이 완료 되었습니다.
바로 사용 가능한 교보e캐시 1,000원 (유효기간 7일)
지금 바로 교보eBook의 다양한 콘텐츠를 이용해 보세요!