Vaccination of Brucella abortus recombinant protein DapB induces CD4+/CD8+ T cells differentiation during Brucella abortus infection in BALB/c mice

• 영문명: Vaccination of Brucella abortus recombinant protein DapB induces CD4+/CD8+ T cells differentiation during Brucella abortus infection in BALB/c mice
• 발행기관: 한국예방수의학회
• 저자명: Tran Xuan Ngoc Huy Ched Nicole Turbela Aguilar Trang Thi Nguyen Said Abdi Salad Hu Jang Lee Jin Hur
• 간행물 정보: 『Journal of Preventive Veterinary Medicine』Vol.47, No.3, 131~136쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2023.09.30

영문 초록

Extensive research and testing continue to be conducted for the development of vaccines targeting zoonotic diseases such as brucellosis. In this study, the potential of the DapB as a recombinant protein vaccine to effectively combat Brucella abortus 544 infection in BALB/c mice was evaluated. Western blotting assay results showed that recombinant protein DapB reacted with Brucella-positive serum, indicating its potential immunoreactivity. In vivo results showed that the peripheral blood CD4+ and CD8+ T cell population significantly increased in the DapB-immunized mice group after the first, second and third blood collection, compared to the control group that received PBS. Additionally, at the fourth blood collection, an increase in CD4+ T cell activation was observed in three vaccination groups compared to PBS negative control group. These results indicate the potential of DapB in stimulating cellular immunity. Fourteen days after infection, the bacterial load in the spleen was evaluated. The reduction in bacterial replication in the spleen by both DapB and RB51 highlights their protective efficacy against Brucella infection. These findings contribute to the ongoing efforts in developing effective vaccines against brucellosis and provide valuable insights for further research in this field.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

키워드

B. abortus DapB gene Subunit vaccine Recombinant protein Adaptive immunity