Suppression of Migration and Invasion by Alnus hirsuta in Human Hepatocellular Carcinoma Cells

• 영문명: Suppression of Migration and Invasion by Alnus hirsuta in Human Hepatocellular Carcinoma Cells
• 발행기관: 한국자원식물학회
• 저자명: 김보람 성수희 Tae-Su Kim 김진호 서찬 이하늘 임수아 김정은 정지민 박정업 최경민 정진우
• 간행물 정보: 『한국자원식물학회지』제36권 제3호, 207~218쪽, 전체 12쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.06.30

영문 초록

Hepatocellular carcinoma (HCC) has a poor prognosis and high metastasis and recurrence rates. Although extracts of Alnus hirsuta (Turcz. ex Spach) Rupr. (AH) have been demonstrated to possess potential anti-inflammatory and anti-cancer activities, the underlying mechanism of AH in HCC treatment remains to be elucidated. We investigated the effects and potential mechanisms of AH on migration and invasion of Hep3B cells. Within the non-cytotoxic concentration range, AH significantly inhibited motility and invasiveness of Hep3B cells in a concentration-dependent manner. Inhibitory effects of AH on cell invasiveness are associated with tightening of tight junctions (TJs), as demonstrated by an increase in transepithelial electrical resistance. Immunoblotting indicated that AH decreased levels of claudins, which form major components of TJs and play key roles in the control and selectivity of paracellular transport. Furthermore, AH inhibited the expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9 and simultaneously increased the levels of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These effects were related to inactivation of the phosphoinositide 3-kinase (PI3K)/AKT pathway in Hep3B cells. Therefore, AH inhibits migration and invasion of Hep3B cells by inhibiting the activity of MMPs and tightening TJs through suppression of claudin expression, possibly by suppressing the PI3K/AKT signaling pathway.

목차

Introduction

Materials and Methods

Results

Acknowledgments

Conflicts of Interest

References

키워드

Alnus hirsuta (Turcz. ex Spach) Rupr. Hepatocellular carcinoma Hep3B cells Invasion Migration PI3K/ AKT