LC-MS/MS를 이용한 건강기능식품 중 키토산 분석법 연구

• 영문명: Analytical Method Development for Determination of Chitosan by LC-MS/MS in Dietary Supplement
• 발행기관: 대한약학회
• 저자명: 박지은(Ji Eun Park) 오미현(Mi Hyune Oh)
• 간행물 정보: 『약학회지』제66권 제4호(2022년), 204~209쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2022.08.30

영문 초록

The Ministry of Food and Drug Safety is amending its test methods for dietary supplements (functional health food) to establish regulatory standards and specifications in South Korea. In this study, we develop analytical test methods to improve the sensitivity and selectivity of the chitosan test method. We develop liquid chromatographic-tandem mass spectrometry for a chitosan analysis using acid hydrolysis to glucosamine. This method can be used to characterize and quantify dietary supplement formulations containing chitosan. The procedure was validated in the 78 ~ 2,500 ng/mL range, and standard calibration curves presented linearity with the coefficient of determination (r²) > 0.999. The limits of detection and quantitation were 51 ng/mL and 154 ng/mL, respectively. The recovery results ranged between 94.8 ~ 102.9% at three different concentration levels with relative standard deviations less than 3.1%, and precision (0.8 ~ 2.5%) was obtained. These are in accordance with the established validation criteria (Association of Official Analytical Chemists). Subsequently, our research provides scientific evidence for amending the chitosan test method for the Health Functional Food Codes.

목차

서 론(Introduction)

방 법(Methods)

결과 및 고찰(Results and Discussion)

결 론(Conclusion)

References

키워드

Chitosan Analytical method LC-MS/MS Dietary supplement