DNA-barcoding을 이용한 제주도 자생 독성 식물 19종의 종 식별 및 데이터베이스 구축

• 영문명: Identification of 19 Species of Poisonous Plants from Jeju Island and Construction of a Database Using DNA-barcoding
• 발행기관: 한국자원식물학회
• 저자명: 권은채(Eunchae Kwon) 김주영(Joo-Young Kim) 장미화(Miwha Chang) 이민지(Minji Lee) 문서현(Seohyun Moon) 이원해(Won-Hae Lee)
• 간행물 정보: 『한국자원식물학회지』제35권 제2호, 346~361쪽, 전체 16쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2022.04.30

영문 초록

Food poisoning accidents caused by poisonous plants occur every year. As certain poisonous plants are mistaken for edible plants causing food poisoning, accurate species identification of poisonous plants is required. DNA barcodes suitable for species identification of poisonous plants and database that can be used for accurate species identification are necessary for their use in forensic cases. In this study, species identification of 19 poisonous plants native to Jeju Island using seven DNA barcodes (trnH-psbA, trnL-trnF, trnL intron, rbcL, matK, ITS1-ITS4, 18S rRNA) was performed to construct a database containing sequence information and DNA barcode universality. trnL-trnF barcode and ITS1-ITS4 barcode were the easiest markers for PCR amplification and sequence retrieval, and the combination of the two barcodes enabled single species identification in 18 out of 19 plants. Therefore, when an investigation of unknown poisonous plants is requested, combination of trnL-trnF and ITS1-ITS4 barcodes is considered as a primary marker for species identification. The database of recommended DNA barcodes for each poisonous plant presented in this study will be helpful in plants poisoning cases.

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서언

재료 및 방법

결과 및 고찰

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사사

Conflicts of Interest

eferences

키워드

Forensic botany Genome database ITS1-ITS4 trnL-trnF