ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification

• 발행기관: 한국육종학회
• 저자명: Jae-Han Son Kyeong-Hoon Kim Sanghyun Shin Hag-Sin Kim Nam-Soo Kim Jong-Nae Hyun Sang-In Shim Choon-Ki Lee Kwang-Geun Park Chon-Sik Kang
• 간행물 정보: 『Plant breeding and biotechnology』Vol.1 No.3, 262~269쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.09.01

영문 초록

Inter simple sequence repeat (ISSR) is a technique that leads to the development of novel specific molecular markers and relationship analysis between species. In this study, 54 ISSRs were used to develop cultivar-specific DNA sequence characterized amplified regions (SCARs) for Korean wheat cultivars (Triticum aestivum). Using nine of 54 UBC ISSR primers, 16 cultivar-specific fragments were detected among 32 wheat cultivars. Of the 16 primers, 10 successfully amplified targets in 32 cultivars and six novel cultivar-specific markers were finally selected. A total of 13 Korean wheat cultivars (Ol, Dahong, Namhae, Jinpum, Anbaek, Jopoom, Jokyung, Sinmichal 1, Jeokjoong, Dajung, Goso and Joah) were classified by newly developed six SCAR markers. Cultivar-specific markers can be used not only for cultivar fingerprinting but also for marker-assisted selection in wheat breeding program.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION

ACKNOWLEDGMENT

REFERENCES

키워드

Molecular marker ISSR SCAR Wheat