Development of Low Gly m Bd 30K (P34) Allergen Breeding Lines Using Molecular Marker in Soybean [Glycine max (L.) Merr.]

• 발행기관: 한국육종학회
• 저자명: Kwang-Ho Jeong Man-Soo Choi Seuk-Ki Lee Min-Jung Seo Tae-Young Hwang Hong-Tai Yun Hong-Sig Kim Jung-Tae Kim Young-Up Kwon Yul-Ho Kim
• 간행물 정보: 『Plant breeding and biotechnology』Vol.1 No.3, 298~306쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.09.01

영문 초록

An increasing production of soybean (Glycine max [L.] Merr.)-based food products may limit dietary choices for soy-allergic individuals. Gly m Bd 30K (P34) is the main seed allergen causing implications in soybean-sensitive patients. The objective of this research was to develop low allergenic soybean lines with molecular marker. The soybean genome assembly specifies that three copy genes of P34 exist in soybean genome. These are Glyma08g12270, which is expressed at significantly higher level over the other two, Glyma08g12280 and Glyma05g29130. Glyma08g12270 was found inactive and was not expressed in low P34 germplasm accessions. Using a co-dominant marker and a polyclonal antibody, polymorphisms and the quantity of protein produced by Glyma08g12270 were analyzed in the F2 and F3 generations obtained by crossing PI567476 and the Korean cultivar Hwanggum. The molecular marker and polyclonal antibody developed in this study could therefore be effectively used for selecting lines that express P34 at low levels. Selected lines could further be used to cross with other null allergenic soybean accessions to breed low allergenic soybean variety.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGMENT

REFERENCES

키워드

Soybean Allergen Gly m Bd 30K (P34) Molecular marker Breeding