A Rapid and Simple Genotyping Method for Various Plants by Direct-PCR

• 발행기관: 한국육종학회
• 저자명: Hyunsik Hwang Shin-Chul Bae Seungbum Lee Yeon-Hee Lee Ancheol Chang
• 간행물 정보: 『Plant breeding and biotechnology』Vol.1 No.3, 290~297쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.09.01

영문 초록

Conventional PCR requires purified DNA molecules as templates. Purification of DNA molecules from a large number of samples is laborious, costly and time-consuming. Therefore, various direct-PCR methods using tissues directly employed as templates have been developed. Using direct-PCR, one can deal with large number of plant samples far more rapidly and efficiently. However, conditions and methods of direct-PCR vary for different plant samples. This is why applications of direct-PCR technology to plant science have been limited. In this study, we have established the appropriate condition for effectively lysing various plant cells and developed the plant cell lysis buffer named ‘Alkaline PEG lysis buffer’ for the direct-PCR. The direct-PCR technology using a newly developed Alkaline PEG lysis buffer successfully amplified different targeted endogenous genes in seven different plant species. This technology is expected to be very useful and effective tool in plant breeding dealing with large number of plants for the selection of targeted traits, markers and pedigrees.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION

ACKNOWLEDGMENT

REFERENCES

키워드

Alkaline lysis buffer Direct-PCR Genotyping Transgenes