학술논문
Cryopreservation of Winter-dormant Apple Buds Using Two-step Freezing
이용수 0
- 영문명
- 발행기관
- 한국육종학회
- 저자명
- Jung Yoon Yi Gi An Lee Sok Young Lee Jong Wook Chung Il Sup Shin
- 간행물 정보
- 『Plant breeding and biotechnology』Vol.1 No.3, 283~289쪽, 전체 7쪽
- 주제분류
- 농수해양 > 기타농수해양
- 파일형태
- 발행일자
- 2013.09.01
4,000원
구매일시로부터 72시간 이내에 다운로드 가능합니다.
이 학술논문 정보는 (주)교보문고와 각 발행기관 사이에 저작물 이용 계약이 체결된 것으로, 교보문고를 통해 제공되고 있습니다.
국문 초록
영문 초록
Winter apple buds germplasm was cryopreserved in the Korean Genebank to back up the genetic resources maintained by field collections. We examined the standard two-step freezing protocol for the cryopreservation of winter buds of apple germplasm developed by National Center for Genetic Resources Preservation (NCGRP) in the USA. This protocol requires desiccation of the stem explants containing a single dormant bud to 30% moisture content, cooling at a rate of 1°C/h to –30°C for 24 hours, followed by rapid immersion in liquid nitrogen. To evaluate the viability of cryopreserved buds after at least 24 hours, the thawed and rehydrated segments are transferred to a greenhouse and used for vegetative propagation by chip budding onto rootstock or by in vitro sprouting. To optimize the protocol for the cryopreservation of winter-dormant apple buds suitable for Korean climate, we tested several treatments, including different degrees of desiccation, pre-freezing temperatures, and pre-freezing rates. The highest regeneration rates were obtained from cryopreserved samples with 35% moisture content, –35°C pre-freezing temperature, and 1°C/h pre-freezing rate. Finally, using our two-step freezing method, 14 accessions of apple germplasm were cryopreserved successfully, with an average regeneration rate of 55.0%.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONCLUSION
ACKNOWLEDGMENT
REFERENCES
해당간행물 수록 논문
- Bioassay for the Response of Resveratrol Transgenic Rice Lines to Bacterial and Fungal Diseases
- ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification
- Bioengineering of Male Sterility in Rice (Oryza sativa L.)
- Selection of Melon Genotypes with Resistance to Fusarium Wilt and Monosporascus Root Rot for Rootstocks
- Cryopreservation of Winter-dormant Apple Buds Using Two-step Freezing
- Isolation and Characterization of Homeodomain-Leucine Zipper III Protein 1 Gene in Poplar
- Development of Low Gly m Bd 30K (P34) Allergen Breeding Lines Using Molecular Marker in Soybean [Glycine max (L.) Merr.]
- Characterization of Expressed Genes Under Ozone Stress in Soybean
- Molecular Genetic Aspects of Self-incompatibility in Brassicaceae
- A Rapid and Simple Genotyping Method for Various Plants by Direct-PCR
- Estimation of Genetic Parameters for Growth Performance and Survival Rate in a Clonal Test of Peronema canescens
참고문헌
관련논문
농수해양 > 기타농수해양분야 BEST
더보기농수해양 > 기타농수해양분야 NEW
- 알코올로 유도된 간손상 모델 HepG2/2E1 세포에서 오리나무 잎 추출물의 간보호효과
- 차광처리가 제비붓꽃의 생장 및 생리적 특성에 미치는 영향
- 꼬리진달래 신초의 폴리페놀 프로파일링과 추출용매에 따른 유용성분 함량 및 항산화 활성 비교
최근 이용한 논문
교보eBook 첫 방문을 환영 합니다!
신규가입 혜택 지급이 완료 되었습니다.
바로 사용 가능한 교보e캐시 1,000원 (유효기간 7일)
지금 바로 교보eBook의 다양한 콘텐츠를 이용해 보세요!